extracellular caspase-8 dependent apoptosis on hela cancer cells and mrc-5 normal cells by icd-85 (venom derived peptides)

نویسندگان

abbas zare mirakabadi dept. of venomous animals and antivenom production, razi vaccine and serum research institute, karaj, iran

ali sarzaeem dept. of venomous animals and antivenom production, razi vaccine and serum research institute, karaj, iran

چکیده

background: our previous studies revealed an inhibitory effect of icd-85 (venom derived peptides) on mda-mb231 and hl-60 cell lines, through induction of apoptosis. the purpose of this study was to investigate apoptosis-induced mechanism on hela and mrc-5 cells by icd-85 through activation of caspase-8. methods: cell viability, cytosolic enzyme lactate dehydrogenase (ldh) and cell morphology were assessed under unexposed and icd-85 exposed conditions.caspase-8 activity was assayed by caspase-8 colorimetric assay kit. results: the results show that inhibitory concentration 50% (ic 50 ) value of icd-85 for hela cells at 24 h was estimated and found to be 25.32±2.15µg/ml. furthermore, treatment of hela cells with icd-85 at concentrations of 1.6×10 and 2.6×10µg/ml did not significantly increase ldh release. morphological changes in hela cells on treatment with icd-85 compared with untreated hela cells consistent with an apoptotic mechanism of cell death, such as cell shrinkage which finally results in the generation of apoptotic bodies. however, when mrc-5 cells were exposed to icd-85, no significant changes in cell morphology and ldh were observed at concentrations below 2.6×10µg/ml. also, the apoptosis-induction mechanism by icd-85 on hela cells was found through activation of caspase-8 and the activity of caspase-8 in hela cells was 1.5 folds more than its activity on mrc-5 cells. conclusion: therefore, the apoptosis-induced mechanisms by icd-85 are through activation of caspase-8 and concerning the least cytotoxic effect on mrc-5 cells, icd-85 may be used as anticancer compound to inhibit growth of cancer cells.

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منابع مشابه

Extracellular Caspase-8 Dependent Apoptosis on HeLa Cancer Cells and MRC-5 Normal Cells by ICD-85 (Venom Derived Peptides)

BACKGROUND Our previous studies revealed an inhibitory effect of ICD-85 (venom derived peptides) on MDA-MB231 and HL-60 cell lines, through induction of apoptosis. The purpose of this study was to investigate apoptosis-induced mechanism on HeLa and MRC-5 cells by ICD-85 through activation of caspase-8. METHODS Cell viability, cytosolic enzyme Lactate Dehydrogenase (LDH) and cell morphology we...

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The biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. The cytotoxicity was evaluated by MTT and LDH assays. The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. The MTT assay showed that ICD-85 NPs could enhance the in-vitro cyt...

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Cytotoxicity of ICD-85 NPs on Human Cervical Carcinoma HeLa Cells through Caspase-8 Mediated Pathway

The biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. The cytotoxicity was evaluated by MTT and LDH assays. The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. The MTT assay showed that ICD-85 NPs could enhance the in-vitro cyt...

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Induction of Apoptosis in Human Leukemia Cell Line (HL60) by Animal’s Venom Derived Peptides (ICD-85)

Our previous studies revealed an inhibitory effect of ICD-85 (Venom derived peptides) on breast cancer cell line MDA-MB231. ICD-85 was also confirmed by in-vivo studies to suppress the breast tumor in mice. However, the exact mechanism of ICD-85 was unknown. Hence, the present study was undertaken to assess the mechanism of ICD-85 effect as an anti-proliferative agent of cancer cells. The effec...

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Induction of Apoptosis in Human Leukemia Cell Line (HL60) by Animal’s Venom Derived Peptides (ICD-85)

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The anti-tumor efficacy of nanoparticulate form of ICD-85 versus free form

Biodegradable polymeric nanoparticles (NPs) have been intensively studied as a possible way to enhance anti-tumor efficacy while reducing side effects. ICD-85, derived from the venom of two separate species of venomous animals, has been shown to exhibit anti-cancer activity. In this report polymer based sodium alginate nanoparticles of ICD-85 was used to enhance its therapeutic effects and redu...

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عنوان ژورنال:
iranian journal of cancer prevention

جلد ۵، شماره ۴، صفحات ۱۹۴-۲۰۲

کلمات کلیدی
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o m e r a s e a c t i v i t y i n h u m a n c a n c e r s . e u r j c a n c e r . 1 9 9 7 ' , 3 3 , 7 8 7 , ' 9 1 . 3 9 . e b i s u n o s ' , ' i n a g a k i t ' , ' k o h j i m o t o y ' , ' o h k a w a t . t h e c y t o t o x i c e f f e c t o f f e r o x a c i n a n d c i p r o f o x a c i n o n t r a n s i t i o n a l c e l l c a r c i n o m a i n v i t r o . c a n c e r . 1 9 9 7 ' , 8 0 , 2 2 6 3 , ' 7 . 4 0 . g ü r b a y a ' , ' g a r r e l c ' , ' o s m a n m ' , ' r i c h a r d m j ' , ' f a v i e r a ' , ' h i n c a l f . c y t o t o x i c i t y i n c i p r o f o x a c i n ' , ' t r e a t e d h u m a n f i b r o b l a s t c e l l s a n d p r o t e c t i o n b y v i t a m i n e . h u m e x p t o x i c o l . 2 0 0 2 ' , 2 1 , 6 3 5 , ' 4 1 . 4 1 . w i j s m a n j a ' , ' d e k a b a n g a ' , ' r i e d e r m j . d i f f e r e n t i a l t o x i c i t y o f r e a c t i v e m e t a b o l i t e s o f c l y n d a m i c i n a n d s u l f o n a m i d e s i n h i v ' , ' i n f e c t e 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u s e s 5 0 p e r c e n t i n h i b i t i o n ( i 5 0 ) o f a n e n z y m a t i c r e a c t i o n . b i o c h e m p h a r m a c o l . 1 9 7 3 ' , 2 2 , 3 0 9 9 , ' 1 0 8 . 4 5 . n e u b i g r r ' , ' s p e d d i n g m ' , ' k e n a k i n t ' , ' c h r i s t o p o u l o s a a . i n t e r n a t i o n a l : u n i o n : o f p h a r m a c o l o g y c o m m i t t e e o n r e c e p t o r n o m e n c l a t u r e a n d d r u g c l a s s i f i c a t i o n . x x x v i i i . u p d a t e o n t e r m s a n d s y m b o l s i n q u a n t i t a t i v e p h a r m a c o l o g y . p h a r m a c o l r e v . 2 0 0 3 ' , 5 5 , 5 9 7 , ' 6 0 6 . 4 6 . f o t a k i s g ' , ' t i m b r e l l j a . i n v i t r o c y t o t o x i c i t y a s s a y s ' , ' c o m p a r i s o n o f l d h ' , ' n e u t r a l r e d ' , ' m t t a n d p r o t e i n a s s a y i n h e p a t o m a c e l l l i n e s f o l l o w i n g e x p o s u r e t o c a d m i u m c h l o r i d e . t o x i c o l o g y l e t t e r s . 2 0 0 6 ' , 1 6 0 , 1 7 1 , ' 7 . 4 7 . b a l d u z z i m ' , ' d i o c i a i u t i m ' , ' d e b e r a r d i s b ' , ' p a r a d i s i s ' , ' p a o l e t t i l . i n v i t r o e f f e c t s o n m a c r o p h a g e s i n d u c e d b y n o n c y t o t o x i c d o s e s o f s i l i c a p a r t i c l e s p o s s i b l y r e l e v a n t t o a m b i e n t e x p o s u r e . e n v i r o n m e n t a l r e s e a r c h . 2 0 0 4 ' , 9 6 , 6 2 , ' 7 1 . 4 8 . s a y e s c m ' , ' g o b i n a m ' , ' a u s m a n k d ' , ' m e n d e z j ' , ' w e s t j l ' , ' c o l v i n v l . n a n o ' , ' c 6 0 c y t o t o x i c i t y i s d u e t o l i p i d p e r o x i d a t i o n . b i o m a t e r i a l s . 2 0 0 5 ' , 2 6 , 7 5 8 7 , ' 9 5 . 4 9 . h u s s a i n s m ' , ' h e s s k l ' , ' g e a r h a r t j m ' , ' g e i s s k t ' , ' s c h l a g e r j j . i n v i t r o t o x i c i t y o f n a n o p a r t i c l e s i n b r l 3 a r a t l i v e r c e l l s . t o x i c o l o g y i n v i t r o . 2 0 0 5 ' , 1 9 , 9 7 5 , ' 8 3 . 5 0 . 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. 5 7 . z h a n g l ' , ' z h u h ' , ' t e r a i s h i f ' , ' d a v i s j j ' , ' g u o w ' , ' f a n z z ' , ' e t a l . a c c e l e r a t e d d e g r a d a t i o n o f c a s p a s e ' , ' 8 p r o t e i n c o r r e l a t e s w i t h t r a i l r e s i s t a n c e i n a d l d 1 h u m a n c o l o n c e l l l i n e . n e o p l a s i a . 2 0 0 5 ' , 7 , 5 9 4 , ' 6 0 2 5 8 . g e r a l d s s ' , ' x i n y i n g j i ' , ' w a n g w ' , ' l e i g h a c a l l a h a n . t h e e x t r i n s i c c a s p a s e p a t h w a y m o d u l a t e s e n d o t o x i n ' , ' i n d u c e d d i a p h r a g m c o n t r a c t i l e d y s f u n c t i o n . j a p p l p h y s i o l . 2 0 0 7 ' , 1 0 2 , 1 6 4 9 , ' 5 7 . 5 9 . l a g u i n g e l m ' , ' s a m a r a r n ' , ' w a n g w ' , ' e l ' , ' d e i r y w s ' , ' c o r n e r g . d r 5 r e c e p t o r m e d i a t e s a n o i k i s i n h u m a n c o l o r e c t a l c a r c i n o m a c e l l l i n e s . c a n c e r r e s e a r c h . 2 0 0 8 ' , 6 8 , 9 0 9 , ' 1 7 . 6 0 . a l f o n s l . a p o p t o s i s a n i n t r o d u c t i o n . r e v i e w a r t i c l e s . b i o e s s a y s . 2 0 0 3 ' , 2 5 , 8 8 8 , ' 9 6 . 6 1 . a r a k i s ' , ' m a s u d a s ' , ' m a e d a h ' , ' y i n g m j ' , ' h a y a s h i h . i n v o l v e m e n t o f s p e c i f i c i n t e g r i n s i n a p o p t o s i s i n d u c e d b y v a s c u l a r a p o p t o s i s i n d u c i n g p r o t e i n 1 . t o x i c o n . 2 0 0 2 ' , 4 0 , 5 3 5 , ' 4 2 . 6 2 . w a i d a j s ' , ' d o w d y s f . m o d u l a t i o n o f c e l l u l a r f u n c t i o n b y t a t m e d i a t e d t r a n s d u c t i o n o f f u l l l e n g t h p r o t e i n s . c u r r p r o t e i n p e p t s c i . 2 0 0 3 ' , 4 , 9 7 , ' 1 0 4 . 6 3 . b a l a s u b a s h i n i m s r i ' , ' k a r t h i g a y a n s ' , ' s o m a s u n d a r a m s t ' , ' b a l a s u b r a m a n i a n t ' , ' r u k k u m a n i r ' , ' v e n u g o p a l p m . f v p e p t i d e i n d u c e s a p o p t o s i s i n h e p 2 a 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